The Department of Pharmacology and Toxicology of the Faculty of Medicine of the Charles University in Pilsen has been long studying the effects of certain environmental pollutants (especially heavy metals, metalloids and substances that would protect the organism from their harmful effects and facilitate their elimination). In the last few years, the experimental program is focused on study of oxidative stress as one of the most important factors of metal toxicity. The staff of the department is looking for substances that would protect organisms from the effects of heavy metals or directly prevent oxidative damage to tissues (eg liver, kidneys). We study chelating agents and especially substances of natural origin (resveratrol, quercetin, curcumin, sesquiterpenic lactones, etc.) Intoxication, oxidative stress and newly changes in the immune system are studied at the cellular and organ levels. The workplace is materially equipped to monitor pharmacokinetic parameters (analytical methods HPLC, AAS), biochemical analyzes of oxidative damage (spectrometry). In the new UNIMEC building, we have created spaces for working with cell lines and for molecular methods (PCR, Western blotting).